Asian Journal of Nuclear Medicine
亞洲核醫學雜志

Volume 2 Number 1, March 2002
Abstract

Leptin受體leptin耐受與肥胖

孫君江1,杜同信1,王自正1,王書奎1,黃敏2
1南京醫科大學附屬南京第一醫院放免中心,南京 210006,中國
2中國藥科大學,南京 210009,中國

目的 通過對肥胖患者外周脂肪組織Leptin受體表達的研究,探討肥胖患者瘦素耐受與瘦素受體的關係。方法 對44例受檢者(排除糖尿病,慢性炎症和惡性腫瘤性疾病)按照其體重指數分為三組,即正常對照組(15例),超重組14例,肥胖組15例。用放射配基結合實驗測定外周脂肪組織中Leptin受體的密度,同時以ELISA試劑盒檢測血清瘦素水平 結果 超重和肥胖患者血清瘦素水平(10.3±4.45和13.2±3.26μg/L)較正常對照組明顯為高(5.51±3.23 μg/L,t=2.064和t=2.341,兩者P <0.05),提示這些患者可能存在瘦素耐受現象。肥胖組和超重組(36.9±5.89和25.3±3.95 fmol/mg膜蛋白)與正常組相比(76.5±35.3 fmol/mg膜蛋白)瘦素受體密度明顯降低(t=2.643和t=3.012, 兩者P<0.01),肥胖組與超重組比較有顯著性差異(t=2.104, P<0.05);三組間的Kd值無差異,表明瘦素受體與配基結合的能力與BMI無關。BMI與瘦素受體密度呈明顯負相關性(r=-0.613,P<0.01), 而血清Leptin與外周Leptin受體無明顯相關性。結論 外周脂肪組織存在Leptin受體表達;低水平leptin受體表達可能與瘦素耐受及肥胖的形成有關。

【關鍵詞】 肥胖;體重指數;瘦素耐受;放射配基

Relationship between peripheral leptin receptor and leptin in obese subjects

Sun Junjiang1, Du Tongxin1, Wang Zizheng1, Wang Shukui1, Huang Min2,
1 Nanjing First Hospital Affiliated to Nanjing Medical University, Nanjing, 210006, China
2 Chinese Pharmaceutical University, Nanjing, 210009, China

Objective To investigate the relationship between leptin resistance and leptin receptor in obese subjects. Methods Forty-four individuals undergoing surgery, exclusive of diabetic mellitus, chronic inflammatory and malignant diseases, were divided into 3 groups according to the body mass index (BMI), normal controls (n=15), weight excess (n=14), and obesity group (n=15). Fasting serum leptin were detected via ELISA kits, leptin receptor (Bmax) in peripheral adipose tissues was detected by radioligand assay. Results Serum leptin levels were higher significantly in weight excess and obesity cases groups (10.3±4.45 and 13.2±3.26 vs 5.51±3.23 mg/L, both P<0.05, respectively) compared with normal control group, suggesting the existence of leptin resistance, while the leptin receptor of the weight excess and obese groups decreased significantly than that of normal control group (36.9±5.89 and 25.3±3.95 vs 76.5±35.3 fmol/mg protein, both P<0.01, respectively), there was no statistical differences for Kd value among three groups. Also, there was a negative correlation between BMI and leptin receptor (r=-0.613, P<0.05), and no significant correlation was found between serum leptin and peripheral leptin receptor. Conclusion: The result suggested that there was expression of leptin receptor in peripheral adipose tissues and low level of leptin receptor expression may contribute to the development of leptin resistance and obesity.

Key Words: Leptin; Receptor; Radioligand; Obesity



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