華中科技大學同濟醫學院附屬同濟醫院核醫學科 武漢 430030,中國。
目的 測定照射後離體腫瘤細胞攝取MIBI能力的變化。方法 將腫瘤細胞按照射劑量(2Gy, 10Gy, 20Gy)與照射後時間(24h, 48h, 72h)進行分組比較。分別測定單位質量腫瘤細胞攝取99mTc-MIBI的放射性計數值(cpm/mg cell protein)，凋亡指數（AI），集落形成率（PE）。結果 不同劑量照射後，腫瘤細胞的99mTc-MIBI攝取值與AI值呈負相關(r=-0.91)，與PE值呈正相關(r=0.86)。結論 不同劑量照射後，腫瘤細胞活力變化可能用99mTc-MIBI作為示蹤劑得到反映。
Monitoring biologic responses of tumour cells after being irradiated with 99mTc-MIBI－A study in vitro
XIA Jinsong, WU Hua, ZHAO
Department of Nuclear Medicine, Tongji Hospital of Tongji Medical University, Wuhan 430030,China.
Abstract : Objective To explore the possibility of 99mTc-MIBI to monitor biologic response of tumor cells after irradiation. Methods To observe the relation between the radiation doses and the uptake levels of 99mTc-MIBI in tumor cells (expressed as cpm/mg cell protein), the cells were irradiated with a single dose of 2Gy, 10Gy and 20Gy, respectively. The uptake of 99mTc-MIBI in each group was determined before and at 24, 48, 72 hours after irradiation, respectively. Apoptosis index (AI), plating efficiency (PE) of tumor cells were simultaneously determined. Results : There was a positive correlation between uptake levels of 99mTc-MIBI and AI (r=-0.91, p<o.o5). A negative correlation was shown between the uptake levels and PE (r=-o.86, p<o.o5). Conclusion It is suggested that 99mTc-MIBI may be used as a tracer to monitor the change of viability state of tumor cells after being irradiated with different doses. (Asian J Nucl Med 2001;1:27-30)
Key Words : 99mTc-MIBI; Tumor cell; Irradiation; Biology effect
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